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Mutation of H143 to an asparagine resulted within the remarkable lack of catalytic exercising as well as a average boost in the K

with various substrate analogs. The reaction system is of curiosity because NagA from bacterial methods is recognized with equally a handful of divalent metallic ions from your Energetic Website-website.

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The metal articles product in addition to the kinetic parameters on your mutants of NagA created for this investigation are made available in Desk 4.

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